Contents

Index

3.3 Reactions in glycolysis

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Glycolysis involves 10 enzymatic reactions, summarized in Figure 3.3-1:

1. The phosphorylation of glucose at position 6 by hexokinase ,
2. the isomerization of glucose-6-phosphate to fructose-6-phospate by phosphohexose isomerase,
3. the phosphorylation of fructose-6-phosphate by phosphofructokinase,
4. the cleavage of fructose-1,6-bisphosphate by aldolase. This yields two different products, dihydroxyacetone phosphate and glyceraldehyde-3-phosphate,
5. the isomerization of dihydroxyacetone phosphate to another molecule of glyceraldehyde phosphate by triose phosphate isomerase,
6. the dehydrogenation and phosphorylation of glyceraldehyde-3-phosphate to 1,3-bis-phosphoglycerate by glyceraldehyde-3-phosphate dehydrogenase,
7. the transfer of the 1-phosphate group from 1,3-bis-phosphoglycerate to ADP to yield ATP by phosphoglycerate kinase, which yields 3-phosphoglycerate,
8. the isomerization of 3-phosphoglycerate to 2-phosphoglycerate by phosphoglycerate mutase,
9. the dehydration of 2-phosphoglycerate to phosphoenolpyruvate by enolase,
10. the transfer of the phosphate group from phosphoenolpyruvate to ADP by pyruvate kinase, to yield another ATP.

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