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WatCut: An on-line tool for restriction analysis, silent mutation scanning, and SNP-RFLP analysis


Welcome to WatCut, an online tool for analyzing DNA sequences with restriction enzymes. WatCut offers the following options:

Restriction analysis (restriction mapping)

Find restriction sites in your DNA sequence. WatCut offers a choice of display formats.

Silent mutation scanning

Use silent mutagenesis to introduce new restriction sites into an oligonucleotide, without changing the encoded protein sequence. This is very useful for screening mutant clones in site-directed mutagenesis experiments.

SNP-RFLP analysis

Quickly find restriction sites suitable for SNP genotyping by PCR-RFLP (restriction length fragment polymorphism).

Check out the Help page for advanced options. Good luck with using WatCut and with your experiments!


Site created and maintained by Michael Palmer, University of Waterloo, Ontario, Canada. Questions / Problems? Send email.
Page last modified: April 17, 2014 . Enzymes as of April 2014, Rebase.